Abstract
The principles of colorimetric detecting oligonucleotides with the help of gold nanospheres and nanorods are discussed. Marker sequences of fragments of HIV-1 genome and Bacillus anthracis are used as models. Experimental data are reported that demonstrate the influence of gold nanorod morphology on the reproducibility of colorimetric tests. A new method is proposed for detecting oligonucleotides based on the application of positively charged gold nanospheres in combination with absorption spectroscopy and dynamic light scattering. Charge reversal of negatively charged gold nanospheres is implemented through the bilayer adsorption of cetyltrimethylammonium bromide molecules. The sensitivity of the proposed method is comparable with the detection of DNA sequences via the colorimetric protocol using nanorods, but it is more simple and stable from the viewpoint of realization. It is shown that the colorimetric tests using gold nanorods and nanospheres do not provide reliable information on the presence of single- and three-base mismatches in target oligonucleotides.
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References
Mirkin, C.A., Letsinger, R.L., Mucic, R.C., and Storhoff, J.J., Nature (London), 1996, vol. 382, p. 607.
Bao, P., Frutos, A.G., Greef, Ch., et al., Anal. Chem., 2002, vol. 74, p. 1792.
Parak, W.J., Gerion, D., Pellegrino, T., et al., Nanotechnology, 2003, vol. 14, p. R15.
Markel, V.A., Shalaev, V.M., Stechel, E.B., et al., Phys. Rev. B: Condens. Matter, 1996, vol. 53, p. 2425.
Khlebtsov, N.G., Dykman, L.A., Krasnov, Ya.M., and Mel’nikov, A.G., Kolloidn. Zh., 2000, vol. 62, p. 844.
Storhoff, J.J., Lazarides, A.A., Mucic, R.C., et al., J. Am. Chem. Soc., 2000, vol. 122, p. 4640.
Lazarides, A.A. and Schatz, G.C., J. Phys. Chem. B, 2000, vol. 104, p. 460.
Khlebtsov, N.G., Melnikov, A.G., Dykman, L.A., and Bogatyrev, V.A., in Photopolarimetry in Remote Sensing, Videen, G., Yatskiv, Ya.S., and Mishchenko, M.I., Eds., Dordrecht: Kluwer, 2004, p. 265.
Demers, L.M., Mirkin, C.A., Mucic, R.C., et al., Anal. Chem., 2000, vol. 72, p. 5535.
Elghanian, R., Storhoff, J.J., Mucic, R.C., et al., Science (Washington, D. C.), 1997, vol. 277, p. 1078.
Sato, K., Hosokawa, K., and Maeda, M., Nucleic Acids Res., 2005, vol. 33, p. D174.
Sato, K., Onoguchi, M., Sato, Y., et al., Anal. Biochem., 2006, vol. 350, p. 162.
Li, H. and Rothberg, L., Anal. Chem., 2005, vol. 77, p. 6229.
Baptista, P., Koziol-Montewka, M., Paluch-Oles, J., et al., Clin. Chem., 2006, vol. 52, p. 1433.
Dai, Q., Liu, X., Coutts, J., et al., J. Am. Chem. Soc., 2008, vol. 130, p. 8138.
He, W., Huang, C.Z., Li, Y.F., et al., Anal. Chem., 2008, vol. 80, p. 8424.
Reynolds, R.A., Mirkin, C.A., and Letsinger, R.L., J. Am. Chem. Soc., 2000, vol. 122, p. 3795.
Mirkin, C.A., Inorg. Chem., 2000, vol. 39, p. 2258.
Doria, G., Franco, R., and Baptista, P., IET Nanobiotechnol., 2007, vol. 1, p. 53.
Li, H. and Rothberg, L., Proc. Natl. Acad. Sci. U. S. A., 2004, vol. 101, p. 14036.
Li, H. and Rothberg, L., J. Am. Chem. Soc., 2004, vol. 126, p. 10958.
Zhang, J., Wang, L., Pan, D., et al., Small, 2008, vol. 8, p. 1196.
Bogatyrev, V.A., Dykman, L.A., Khlebtsov, B.N., and Khlebtsov, N.G., Opt. Spektrosk., 2004, vol. 96, p. 139.
Sau, T.K. and Murphy, C.J., Langmuir, 2004, vol. 20, p. 6414.
Xu, X. and Cortie, M., Adv. Funct. Mater., 2006, vol. 16, p. 2170.
Ratto, F., Matteini, P., Rossi, F., and Pini, R., J. Nanoparticle Res., 2010, vol. 12, p. 2029.
Frens, G., Nature Phys. Sci., 1973, vol. 241, p. 20.
Takahashi, H., Niidome, Y., and Yamada, S., Chem. Commun., 2005, p. 2247.
Derjaguin, B.V., Churaev, N.V., and Muller, V.M., Surface Forces, New York: Consultants Bureau, 1987.
Bogatyrev, V.A., Dykman, L.A., Krasnov, Ya.M., et al., Kolloidn. Zh., 2002, vol. 64, p. 745.
Khlebtsov, N.G., Bogatyrev, V.A., Dykman, L.A., et al., J. Quant. Spectrosc. Radiat. Transfer, 2004, vol. 89, p. 133.
Alekseeva, A.V., Bogatyrev, V.A., Khlebtsov, B.N., et al., Kolloidn. Zh., 2006, vol. 68, p. 725.
Khlebtsov, B.N., Khanadeev, V.A., and Khlebtsov, N.G., J. Phys. Chem. C, 2008, vol. 112, p. 12760.
Shulepov, S.Yu. and Frens, G., J. Colloid Interface Sci., 1996, vol. 182, p. 388.
Jain, P.K., Eustis, S., and El-Sayed, M.A., J. Phys. Chem. B, 2006, vol. 110, p. 18243.
Oldenburg, S.J., Genick, Ch.C., Clark, K.A., and Schultz, D.A., Anal. Biochem., 2002, vol. 309, p. 109.
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Original Russian Text © T.E. Pylaev, V.A. Khanadeev, B.N. Khlebtsov, L.A. Dykman, V.A. Bogatyrev, N.G. Khlebtsov, 2011, published in Kolloidnyi Zhurnal, 2011, Vol. 73, No. 3, pp. 364–374.
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Pylaev, T.E., Khanadeev, V.A., Khlebtsov, B.N. et al. Effects of shape and charge of colloidal gold nanoparticles in colorimetric determination of DNA sequences. Colloid J 73, 368–377 (2011). https://doi.org/10.1134/S1061933X11020104
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DOI: https://doi.org/10.1134/S1061933X11020104